抗連接子（linker）單抗帶來了通用型CAR檢測的“希望之光”。大多數(shù)CAR包含單鏈可變片段（scFv），Carvykti除外（具有獨特的納米抗體結(jié)構(gòu)VHH）。scFv帶有柔性Whitlow 218或G4S連接子。連接子廣泛應(yīng)用于CAR構(gòu)建中，標(biāo)準(zhǔn)化的氨基酸序列提供明確的靶標(biāo)。不管CAR-T識別的腫瘤抗原是什么（如CD19、BCMA、Claudin18.2、CD22），只要其結(jié)構(gòu)中含有連接子，抗連接子抗體均能準(zhǔn)確檢測。與依賴scFv抗原特異性檢測方法相比，抗連接子抗體顯著提升檢測的通用性和一致性。

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 全場景通用

不管CAR靶向那種抗原，只有含有Whitlow218 Linker序列，就可用該類抗體進行檢測，告別“一CAR一試劑”的定制化困境。

 多標(biāo)記選擇

義翹神州抗Whitlow 218 Linker抗體可以用于FITC、PE、APC、AF488、AF647、Biotin標(biāo)記的流式細胞術(shù)。

 特異性超高

自研開發(fā)的兔單克隆抗體，流式細胞術(shù)檢測顯示，PBMC非特異結(jié)合低于0.5%。

 體內(nèi)外通用

不受實驗環(huán)境限制，可用于體外檢測CAR-T細胞轉(zhuǎn)染效率，也可在體內(nèi)動態(tài)追蹤CAR-T細胞擴增效果。

 研發(fā)速度加快

省去定制化檢測試劑的開發(fā)與驗證步驟，CAR設(shè)計、表達驗證周期更快速。

驗證數(shù)據(jù)

 高效識別CAR陽性細胞

Flow cytometric analysis of anti-ERBB2 CAR expression on anti-ERBB2 CAR-transfected 293T cells. Cells were stained with PE-conjugated anti-Whitlow 218 linker Antibody (Cat: 111774-R0013-P). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Non-transduced 293 cells were used as a control (right).

 高特異性結(jié)合

Binding activity of FITC-conjugated anti-Whitlow 218 linker Antibody to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and FITC-conjugated anti-Whitlow 218 linker Antibody (Cat: 111774-R0013-F) and detected by flow cytometry. FITC signal was used to evaluate the non-specific binding activity to PBMCs.

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義翹神州通用型Whitlow 218 Linker兔單抗，高效助力CAR流式檢測，歡迎掃碼查看更多相關(guān)信息>>