p38是絲裂原活化蛋白激酶MAPK家族的重要成員。p38的激活需要Thr180、Tyr182位點(diǎn)發(fā)生磷酸化，在細(xì)胞增殖、代謝、凋亡等方面發(fā)揮重要作用，與多種腫瘤發(fā)展相關(guān)。

義翹神州通過其自有的流式單B技術(shù)制備了一系列磷酸化兔單抗。磷酸化兔單抗經(jīng)過多種實(shí)驗(yàn)技術(shù)驗(yàn)證其特異性，應(yīng)用廣泛，有效保證了抗體的批間差、穩(wěn)定性。

下面以Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Antibody, Rabbit Monoclonal （Cat: 110435-R0004）為例為大家介紹：

• WB應(yīng)用（多肽競爭特異性驗(yàn)證）

Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p38 MAPK (Thr180, Tyr182) rabbit monoclonal Antibody (Cat# 110435-R0004) at 1:1000 dilution.

• IHC應(yīng)用（磷酸酶特異性驗(yàn)證）

Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-p38 MAPK (Thr180-Tyr182) Antibody, Rabbit Monoclonal (Cat# 110435-R0004) at 1:500 dilution.

• IF應(yīng)用（磷酸酶特異性驗(yàn)證）

Immunofluorescence staining of Phospho-p38 MAPK (Thr180, Tyr182) in serum-starved NIH-3T3 cells, untreated (left), treated with UV (30mins) (middle) or treated with UV (30mins) and lambda phosphatase (right). Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse Phospho-p38 MAPK (Thr180, Tyr182) monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was mainly localized to Nucleus.

• 穩(wěn)定性測試（加速實(shí)驗(yàn)）

Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Antibody, Rabbit Monoclonal (Cat: 110435-R0004) was placed in 25℃ for 8 days, in 25℃ for 16 days, in 37℃ for 3.5 days, in 37℃ for 7 days respectively. The cell lysates were serum-starved NIH-3T3, untreated or treated with UV (30 min). The antibody after accelerated by different temperature still showed significant specificity.

免費(fèi)試用活動(dòng)進(jìn)行中！

為了助力研究，現(xiàn)在義翹神州為廣大研究者提供大量磷酸化兔單抗免費(fèi)試用，誠邀大家掃碼領(lǐng)取。與此同時(shí)，義翹神州也可為前沿研究者開發(fā)新靶點(diǎn)磷酸化抗體，拓展生物學(xué)研究的新邊界。

立即掃碼申請磷酸抗體試用裝！