p38是絲裂原活化蛋白激酶MAPK家族的重要成員。p38的激活需要Thr180、Tyr182位點發生磷酸化，在細胞增殖、代謝、凋亡等方面發揮重要作用，與多種腫瘤發展相關。

義翹神州通過其自有的流式單B技術制備了一系列磷酸化兔單抗。磷酸化兔單抗經過多種實驗技術驗證其特異性，應用廣泛，有效保證了抗體的批間差、穩定性。

下面以Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Antibody, Rabbit Monoclonal （Cat: 110435-R0004）為例為大家介紹：

• WB應用（多肽競爭特異性驗證）

Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p38 MAPK (Thr180, Tyr182) rabbit monoclonal Antibody (Cat# 110435-R0004) at 1:1000 dilution.

• IHC應用（磷酸酶特異性驗證）

Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-p38 MAPK (Thr180-Tyr182) Antibody, Rabbit Monoclonal (Cat# 110435-R0004) at 1:500 dilution.

• IF應用（磷酸酶特異性驗證）

Immunofluorescence staining of Phospho-p38 MAPK (Thr180, Tyr182) in serum-starved NIH-3T3 cells, untreated (left), treated with UV (30mins) (middle) or treated with UV (30mins) and lambda phosphatase (right). Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse Phospho-p38 MAPK (Thr180, Tyr182) monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was mainly localized to Nucleus.

• 穩定性測試（加速實驗）

Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Antibody, Rabbit Monoclonal (Cat: 110435-R0004) was placed in 25℃ for 8 days, in 25℃ for 16 days, in 37℃ for 3.5 days, in 37℃ for 7 days respectively. The cell lysates were serum-starved NIH-3T3, untreated or treated with UV (30 min). The antibody after accelerated by different temperature still showed significant specificity.

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為了助力研究，現在義翹神州為廣大研究者提供大量磷酸化兔單抗免費試用，誠邀大家掃碼領取。與此同時，義翹神州也可為前沿研究者開發新靶點磷酸化抗體，拓展生物學研究的新邊界。

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