轉運RNA (tRNA) 是生物體內分布最廣泛、含量最豐富的非編碼小RNA分子。它攜帶并轉運氨基酸，參與蛋白翻譯，是連接mRNA與蛋白質的重要橋梁。細胞增殖[1]、分化[1, 2]和凋亡[3]等一系列生物學過程都伴隨著tRNA水平的變化。反之，tRNA表達譜的改變也會影響細胞發育過程中的命運抉擇。表達失調的tRNA可以促進腫瘤的發生和癌癥進程[2, 4-11]。另外，許多其它疾病比如II型糖尿病[12]、亨廷頓癥[13]以及HIV感染[14]都出現了tRNA表達與分布紊亂。tRNA研究已逐漸成為生物學過程和疾病研究的重要組成部分。

Arraystar最新發布了市場上首款專注于tRNA研究的PCR芯片——nrStar™ Human tRNA Repertoire PCR Array。該款芯片可同時檢測66個細胞核tRNA和22個線粒體tRNA，覆蓋了tRNA權威數據庫GtRNAdb和tRNAdb中的所有反密碼子，方便客戶快速地進行tRNA表達譜分析，為下一步的密碼子偏好性、蛋白翻譯效率和準確性、細胞動力學以及病毒感染的細胞嗜性等研究提供重要線索，是進行tRNA研究必不可少的工具。

tRNA存在種類繁多的修飾，對其發揮功能十分關鍵。然而，這些修飾尤其是甲基化修飾會嚴重阻礙反轉錄的進行，導致cDNA合成終止或堿基錯配。為此，Arraystar專門開發了針對tRNA的反轉錄試劑盒(rtStar™ tRNA-optimized First-Strand Synthesis Kit)。該試劑盒采用了一種高效的RNA去甲基化酶AlkB，能夠有效的去除tRNA上的甲基化修飾，極大的提高cDNA合成質量。與此試劑盒組合使用，研究人員能夠獲得更為真實可靠的tRNA表達變化，為研究蛋白質組或tRNA來源片段（tRFs）提供重要信息。

為了保證數據可信度，芯片還包含了3個看家小RNA作為內參，以及3個質控對照RNA Spike-in、PCR陽性對照（PPC）和基因組DNA對照（GDC）,分別用于監測cDNA合成質量、PCR效率和基因組DNA污染。

在后續研究中，敲低tRNA[15] 或過表達tRNA [2, 16, 17]可以進一步揭示特定tRNA在細胞生命活動中的作用。其它用于非編碼RNA研究的相關技術也適用于tRNA的后續研究。

產品列表

芯片特點

• 囊括GtRNAdb和tRNAdb數據庫中所有的細胞核與線粒體反密碼子
• 伴隨的去甲基化處理使得檢測結果更加真實可靠
• 所有引物均在多種細胞和組織中通過驗證
• 即拆即用型384孔板，幾小時內便可得到結果

工作流程

 
適用實時定量熒光PCR儀：

ABI ViiA™ 7，ABI 7500 & ABI 7500 FAST，ABI 7900HT，ABI QuantStudio™ 6 Flex Real-Time PCR system，ABI QuantStudio™ 7 Flex Real-Time PCR system，ABI QuantStudio™ 12K Flex Real-Time PCR System，Bio-Rad CFX384，Bio-Rad iCycler & iQ Real-Time PCR Systems，Eppendorf Realplex，QIAGEN Rotor Gene Q100，Roche LightCycler 480，Stratagene Mx3000，Roche LightCycler 480

數據庫

tRNA反密碼子信息(GtRNAdb和tRNAdb數據庫)：

立即索取nrStar Human tRNA Repertoire PCR Array的詳細資料

參考文獻
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